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Offer 219 out of 336 from 15/09/23, 08:01

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Tech­ni­sche Uni­ver­sität Ber­lin - Faculty III - Institute of Biotechnology / Bioprocess Engineering

2 Positions - Research Assistant - salary grade 13 TV-L Berliner Hochschulen

part-time employment may be possible

Working field:

Implementation of a 2-D HPLC system and a QTOF mass spectrometry in the environment of an automated bioprocess development lab.

Here we advertise two positions to complete our KIWI-biolab team with bioanalytical experts that are competent in the analysis of biomolecules by liquid chromatography and mass spectrometry.

The project aims for the smart integration of LC/MS into our high throughput bioprocess development facility (KIWI-biolab) and to develop standardized workflows and a shared database that would enable a holistic optimisation of bioprocesses across units and scales.
The focus is on a fully automated and properly integrated analysis workflow of LC/MS devices that offer:
(i) optimal performance for the respective scientific question,
(ii) High throughput solutions to solve the current bottlenecks with adequate LC/MS performance
criteria,
(ii) a well-structured and standardized strategy for data management and sharing according to FAIR data principles.

The feasibility and potential of the integrated use of LC/MS to follow the process dynamics in parallel bioreactors and to detect critical quality attributes (CQAs) is demonstrated at two exemplary levels:
(1) Metabolomic approach: Integrated HT analysis of medium components and metabolites adopting our current offline analytical LC/MS methods for
(i) short-chain carboxylic acids,
(ii) quantitative extracellular and intracellular amino acid analysis,
(iii) nucleotides, including cellular alarmons such as cyclic AMP and ppGpp.
(2) Proteomic approach:
(i) analysis of secreted and released host cell proteins (HCPs) in the cultivation medium,
(ii) proteome analysis for the (semi)quantitative analysis of marker proteins,
(iii) product- and process relevant interactome analysis (subunit interaction, inclusion body structure and composition).

The project will be conducted in close cooperation with other scientists in the KIWI-biolab which are specialists on automation, robotics, FAIR data management and mathematical modelling.

Among the main tasks are:
  • Integration of an 1290 Infinity II Bio 2D-LC / 6546 LC/MS QTOF system from Agilent into the KIWI-biolab environment including the implementation of the FAIR data management
  • Adaptation and development of the methods mentioned above for the analysis of fermentation media, amino acids, nucleotides and proteins
  • Implementation of the sample preparation and extraction methods on a liquid handling station
  • Practical advisor/supervison of Bachelor and Master theses

Requirements:

  • Successfully completed university degree (Master, Diploma or equivalent) in the field of biotechnology, chemistry or a related area
  • Proven experience in the area of bioanalytics and a focus on liquid chromatography and mass spectrometry advantage.
  • A finalised PhD degree is an advantage but not necessary
  • Independent scientific work in the area of analytical biotechnology or chemistry with a focus on the HPLC analysis and/or mass spectrometry of at least one of the following substance classes: sugars, amino acids, nucleosides/nucleotides, proteome
  • Experience in the analysis of pharmaceutically relevant critical protein product quality attributes (glycosylation, disulfide bind pattern, MS-based interaction based research) is an advantage
  • The candidate should be open-minded
  • The ability to teach in German and/or in English is required; willingness to acquire the respective missing language skills
  • The willingness to actively participate in scientific events is a pre-requisite

How to apply:

Please send your application with the reference number and the usual documents (combined in a single pdf file, max. 5 MB) by email to Prof. Dr. Peter Neubauer (bioprocess-tb-office@win.tu-berlin.de).

By submitting your application via email you consent to having your data electronically processed and saved. Please note that we do not provide a guarantee for the protection of your personal data when submitted as unprotected file. Please find our data protection notice acc. DSGVO (General Data Protection Regulation) at the TU staff department homepage: https://www.abt2-t.tu-berlin.de/menue/themen_a_z/datenschutzerklaerung/ or quick access 214041.

To ensure equal opportunities between women and men, applications by women with the required qualifications are explicitly desired. Qualified individuals with disabilities will be favored. The TU Berlin values the diversity of its members and is committed to the goals of equal opportunities.

Technische Universität Berlin - Der Präsident - , Fakultät III, Institut für Biotechnologie, FG Bioverfahrenstechnik, Prof. Dr. Peter Neubauer, Sekr. ACK24, Ackerstraße 76, 13355 Berlin